ywkB [2019-07-15 08:48:53]

unknown

locus: BSU_37040

pI: 7.11

mw: 33.49 kDa

protein length: 319 aa

gene length: 960 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

Gene

Coordinates: 3,799,377 3,800,336

The protein

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|4F30A4412768E6313F57BD56C4EF5079F500E0C4|maeA]-[gene|1E602BCB2A416CDBF2B5007765EC9628123FE979|ywkB]
  description: [Pubmed|12949160]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12949160], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|689CF50691E77E5A40437EF1E6B5083AF7CFCE08|MalR]: activation, [Pubmed|12949160], in [regulon|689CF50691E77E5A40437EF1E6B5083AF7CFCE08|MalR regulon]

  regulation

  • expressed in the presence of malate ([protein|search|MalR]) [Pubmed|12949160]

  additional information

  • the mRNA is substantially stabilized upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]

Biological materials

Mutant

• MGNA-B656 (ywkB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1655 NBRP B. subtilis, Japan]
• BKE37040 ([gene|1E602BCB2A416CDBF2B5007765EC9628123FE979|ywkB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAATATTCTCCTCCAAAT, downstream forward: _UP4_TAACAAAAAAAGCTCCCTTT
• BKK37040 ([gene|1E602BCB2A416CDBF2B5007765EC9628123FE979|ywkB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAATATTCTCCTCCAAAT, downstream forward: _UP4_TAACAAAAAAAGCTCCCTTT

References

• 12949160,21815947